Microscopy, Chromatographic & Spectrophotometric Analysis
THS has the capability to provide the below mentioned services for testing of nutraceuticals, herbal, dietary or nutritional supplements companies in the global market. We at THS with the help of modern phytoanalytical chromatographic method of analysis develop complete analytical profile (Chemical fingerprinting) of herbals.
Microscopy
Nowadays, microscopic evaluation is indespensible in the intial identification of herbs or detection of foreign matter and adulterants as qualtiy control of herbal drugs has been based on apperance. Microscopic examination focuses on anatomical structures in the plant material that are visible only with the help of a microscope.

Spectroscopical methods
  • Ultra Violet- Visible Spectroscopy (UV- Vis)
  • UV which is also termed as visible spectrophotometer allows simple and cost effective analysis of samples containing chromosphores or are coloured and show absorption in the UV visible region.

  • Infra Red Spectroscopy (IR)
  • IR is one of the most common spectroscopic technique used by chemist. The main reason IR is used is to determine the chemical functional groups in the wide range of sample types such as gases, liquid and solid.

  • Fourier Transform Infrared Spectroscopy (FTIR)
  • FTIR, is mainly used to identify organic materials. FTIR analysis results in an absorption spectra which provides information about the chemical bonds and molecular structure of a material. Our experience and expertise in FTIR (Fourier Transform Infrared) spectroscopy can produce accurate and reliable results across a diverse and expansive range of applications. FTIR helps in identification of unknown materials, also can determine the quality or consistency of a sample along with the amount of components in a mixture

  • Atomic Absorption Spectrophotometry (AAS)
  • AAS is mainly used when natural products have metals presents in trace quantities or when quantitative analysis is to be perfromed.

  • Nuclear Magnetic Resonance Spectroscopy (NMR)
  • Nuclear Magnetic Resonance (NMR) Spectroscopy is one of the modern techniques to determine the structure of the orgainc compounds in the natural samples. It is used to determine the purity as well as to identify contaiminants in food, cosmetics or medicines.
Chromatographic methods
  • Thin Layer Chromatography (TLC)
  • TLC (Thin Layer Chromatography) is extensively used in herbal authentication and mentioned in pharmacopoeial monographs. TLC seperates mixtures of compounds which can be compared with authentic sample or pure reference compounds.

  • Column Chromatography (CC)
  • Column Chromatography is another common and useful separation technique in organic chemistry. Column chromatography allows us to separate and collect the compounds individually. The applications of this technique are wide reaching and cross many disciplines including biology, biochemistry, microbiology and medicine.

  • High-performance liquid chromatography (HPLC)
  • High-performance liquid chromatography (HPLC) is another type of chromatography widely used in the authentication and analysis of herbal substances. It is also used to determine purity, compare claims by raw material suppliers, determine degradation of actives and test shelf life of Nutraceuticals and Dietary Supplements as well as verify the Nutritional Label of a Food or Herbal product.

  • High Performance Thin Layer Chromatography (HPTLC)
  • HPTLC (High Performance Thin Layer Chromatography) finger printing, is commonly used in the pharmaceutical and herbal industry for the identification and detection of adulterants in herbal products. It is also used in quality control of herbls and dietary supplements.
Other combined methods
  • Liquid chromatography Mass Spectroscopy (LC-MS)
  • Recently, Liquid chromatography Mass Spectrroscopy has become the preferable method in many stages of drug development as it combines HPLC with MS.

  • Gas chromatography-mass spectroscopy (GC-MS)
  • Gas chromatography-mass spectroscopy (GC-MS) is one of the analytical techniques which is a method of analysing mixture of components to determine the quantities (concentration) of each of the components.